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背景:帕金森病治疗方法有多种,多巴胺替代疗法、外科靶点永久毁损、脑深部核团高频电刺激术、脑移植和基因治疗。这些外科及药物治疗都有一些不足及难以弥补的缺陷,国内外学者将多巴胺能神经元保护剂的研究和应用提到了重要位置。目的:观察评价预先应用谷氨酸受体拮抗剂犬尿烯酸对黑质多巴胺能神经元及神经传导纤维损伤的保护性作用。设计:采用随机对照单盲实验研究。地点和材料:研究地点为解放军总医院神经外科实验室;实验动物选取雌性SD大鼠(取自解放军总医院动物饲养室,40只,体质量210～240g,平均228g)。干预:雌性SD大鼠40只,随机分为4组,每组10只,应用江湾I型C立体定向仪,在单侧黑质致密部及中脑被盖腹侧部,分别注射生理盐水,犬尿烯酸,犬尿烯酸加6-羟多巴胺,6-羟多巴胺。注射药物3d后,进行症状观察,2周后处死大鼠。切片HE染色观察黑质细胞的形态特点,冷冻切片免疫组化特殊染色观察TH阳性细胞及TH阳性纤维着色情况,实验数据分别采用方差分析以及秩和检验进行统计分析。主要观察指标:切片HE染色观察黑质细胞的形态特点,冷冻切片免疫组化特殊染色观察TH阳性细胞数及TH阳性纤维着色等级。结果:正常黑质细胞体形较大,富含黑色素颗粒,可见尼氏体。数据统计分析结果提示犬尿烯酸组与生理盐水组之间差异无显
Background: There are a variety of treatments for Parkinson’s disease, dopamine replacement therapy, permanent destruction of surgical targets, high-frequency electrical stimulation of deep brain nuclei, brain transplantation and gene therapy. These surgical and medical treatment have some shortcomings and irreparable defects, domestic and foreign scholars dopaminergic neuron protective agent research and application of the important position mentioned. OBJECTIVE: To observe and evaluate the protective effect of kynurenine, a glutamate receptor antagonist, on the nigral dopaminergic neurons and nerve-derived fibers. Design: Randomized controlled single-blind experimental study. Location and Materials: The study site was the Neurosurgery Laboratory of Chinese PLA General Hospital. The female SD rats were selected as experimental animals (40 from the Animal Husbandry Department of the People’s Liberation Army General Hospital with an average body weight of 210-240 g). Intervention: Forty female Sprague-Dawley rats were randomly divided into 4 groups with 10 rats in each group. The corpus striatum of unilateral substantia nigra pars compacta and midbrain were injected with normal saline , Kynurenic acid, kynurenic acid plus 6-hydroxydopamine, 6-hydroxydopamine. After injection of the drug for 3 days, the symptoms were observed and the rats were sacrificed after 2 weeks. The morphological characteristics of nigral cells were observed by HE staining. Immunohistochemical staining was used to observe the staining of TH positive cells and TH positive fibers in frozen section. The experimental data were analyzed by variance analysis and rank sum test, respectively. MAIN OUTCOME MEASURES: Specimens were stained with hematoxylin and eosin (HE) to observe the morphological characteristics of nigral cells. Immunohistochemical staining was used to observe the number of TH positive cells and the staining level of TH positive fibers. Results: The normal normocytes were large in shape and rich in melanin granules, showing Nissl. Data statistical analysis showed no significant difference between kynurenic acid group and saline group