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目的:探讨细胞质胸苷激酶(TK1)在宫颈鳞癌的表达及其临床意义。方法:制备40例维吾尔族妇女子宫颈癌及正常宫颈组织中总RNA,用RT-PCR方法分析TK1基因mRNA的表达水平,用免疫组化(SP)法检测其相应蛋白表达。结果:(1)维吾尔族妇女宫颈癌组织中TK1 mRNA及蛋白阳性检出率分别为67.5%及60.5%,对照组宫颈组织中检出率为26.7%及8.3%。宫颈癌组织中TK1 mRNA及蛋白阳性表达明显高于对照组的宫颈组织(P<0.05);(2)在宫颈癌组织中TK1 mRNA及蛋白表达:不同临床分期及分化程度之间无显著差异(P>0.05),但是,有淋巴结转移患者组织中阳性表达明显高于无淋巴结转移者,肿瘤直径≥4cm患者阳性表达明显高于肿瘤直径<4cm的患者(P<0.05)。结论:TK1在维吾尔族妇女宫颈鳞癌中高表达,对宫颈癌的诊断有重要的参考价值,TK1高表达可能与宫颈癌的进展及预后有关。
Objective: To investigate the expression of cytoplasmic thymidine kinase (TK1) in cervical squamous cell carcinoma and its clinical significance. Methods: The total RNA was extracted from 40 Uygur women with cervical cancer and normal cervical tissue. The expression of TK1 gene mRNA was analyzed by RT-PCR and the expression of TK1 protein was detected by immunohistochemistry (SP). Results: (1) The positive rates of TK1 mRNA and protein in cervical cancer tissues of Uighur women were 67.5% and 60.5% respectively, while those in control group were 26.7% and 8.3% respectively. The expression of TK1 mRNA and protein in cervical cancer tissues was significantly higher than that in the control group (P <0.05). (2) The expression of TK1 mRNA and protein in cervical cancer tissues: there was no significant difference between different clinical stages and differentiation However, the positive expression of lymph node metastasis was significantly higher in patients with no lymph node metastasis, the tumor diameter ≥ 4cm patients was significantly higher than the tumor diameter <4cm patients (P <0.05). Conclusion: TK1 is highly expressed in Uighur women with cervical squamous cell carcinoma and has important reference value for the diagnosis of cervical cancer. The high expression of TK1 may be related to the progression and prognosis of cervical cancer.