论文部分内容阅读
目的通过建立荷瘤大鼠胶质瘤模型,探讨极低频电磁场暴露对大鼠脑内胶质细胞瘤的影响,为胶质瘤的实验性治疗提供理论依据。方法将1×106/ml的C6细胞悬液10μl注入大鼠颅内,建立大鼠胶质瘤动物模型,随机分成实验组、对照组各15只,3d后实验组每天于磁场中暴露3h,连续1周,对照组不暴露于磁场,每天不定时观察大鼠精神、神经功能(偏瘫情况)、存活期以及对死亡大鼠的尸体解剖病理切片进行分析。结果对照组接种肿瘤后16-27d全部死亡,平均存活22d,实验组接种肿瘤后21-33d全部死亡,平均存活26d,组间有显著差异(P<0.05),两组死亡大鼠解剖均发现颅内肿瘤生长明显,但病理切片并未能证实两组胶质瘤有形态学差异。结论极低频电磁场暴露可以延长荷瘤大鼠生存时间,但是并不能治愈胶质细胞瘤,提示低强度的极低频电磁场短时间的暴露对胶质瘤患者是无毒性也无治疗作用的,而长时间低强度的极低频电磁场暴露可能对胶质瘤患者有治疗作用。
OBJECTIVE: To establish a tumor-bearing rat glioma model to explore the effect of ultra-low frequency electromagnetic field exposure on glioma in rat brain and to provide a theoretical basis for the experimental treatment of glioma. Methods 10μl of 1 × 106 / ml C6 cell suspension was injected intracranially to establish the animal model of glioma in rats. The rats were randomly divided into experimental group and control group with 15 rats in each group. After 3 days, the experimental group was exposed to magnetic field for 3 hours, The rats in the control group were not exposed to the magnetic field for one week. The rats’ spirits, neurological function (hemiplegia), survival period and the autopsy pathological sections of the deceased rats were analyzed daily. Results All the mice died after 16-27 days of vaccination in the control group, with an average survival of 22 days. All mice died in 21-33 days after vaccination in the experimental group, with an average survival of 26 days. There was significant difference between the two groups (P <0.05) Intracranial tumor growth, but the pathological section did not confirm the two groups of gliomas have morphological differences. Conclusion Electromagnetic field exposure in very low frequency can prolong the survival time of tumor-bearing rats, but it can not cure glioma. It suggests that the short-term exposure of low-intensity EMF is non-toxic and non-therapeutic in patients with glioma. Low-intensity low-frequency electromagnetic field exposure may have a therapeutic effect on glioma patients.