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目的：探讨多聚（二磷酸腺苷－核糖）聚合酶（ＰＡＲＰ）在ＯＮＯＯ－致豚鼠气管高反应性中的作用。 方法；建立离体豚鼠气管条对组胺反应的浓度反应曲线，观察ＰＡＲＰ抑制剂３－氨基苯甲酸胺（３－ＡＢ）对过氧亚 硝基阴离子（ＯＮＯＯ－）诱导豚鼠气管反应性变化的影响。结果：ＯＮＯＯ－（０．５ ｍｍｏｌ／Ｌ）引起豚鼠气管上皮细胞明 显受损，气管条对组胺的反应性及敏感性明显增高，３－ＡＢ（１ｍｍｏｌ／Ｌ或５ ｍｍｏｌ／Ｌ）逆转了 ＯＮＯＯ－的上述作用；３ －ＡＢ（５ ｍｍｏｌ／Ｌ）本身对正常气管条收缩反应无明显影响。结论：ＰＡＲＰ介导了 ＯＮＯＯ－所引起的气道上皮细胞 的损伤和气道高反应性的形成，提示通过抑制ＰＡＲＰ的过度活化可为防治哮喘气道高反应性提供新策略。
Objective: To investigate the role of poly (ADP-ribose) polymerase (PARP) in the bronchial hyperresponsiveness induced by ONOO-induced guinea pigs. Methods The concentration response curve of isolated guinea pig tracheal strips to histamine reaction was established. The effect of PARP inhibitor 3-aminobenzamide (3-AB) on the changes of trachea in guinea pigs induced by peroxynitrite anion (ONOO-) was observed influences. Results: ONOO- (0.5 mmol / L) significantly damaged the tracheal epithelial cells of guinea pigs, and the reactivity and sensitivity of tracheal strips to histamine were significantly increased. 3-AB (1 mmol / L or 5 mmol / L) ONOO-; 3-AB (5 mmol / L) itself had no significant effect on the contraction of normal tracheal strips. CONCLUSION: PARP mediates ONOO-induced airway epithelial cell injury and airway hyperresponsiveness, suggesting that PARP may provide a new strategy to prevent and treat airway hyperresponsiveness by inhibiting PARP activation.