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用激光扫描共聚焦显微镜及荧光漂白后回复技术，观察HDL，LDL及维生素对培养的人血管内皮细胞间隙连接通讯功能的影响。结果：培养条件下的人血管内皮细胞之间可形成功能性的间隙连接。LDL组的荧光回复率分别为6．691±2．634，4．153±2．125，2.441±0.720，与对照组比较及两两比较，有显著性差异（P＜0.05）；HDL组及维生素C组的荧光回复率与对照组比较，无显著性差异；LDL＋HDL组的荧光回复率分别为3．500±0.890，4．339±1．126，5．243±1．278，与对照组比较及两两比较，有显著性差异（P＜0．05）；LDL＋维生素C组的荧光回复率分别为3．959±1．088，4．974±1．194，8．673±2．488，与对照组比较及两两比较，有显著性差异（P＜0．05）。以上结果提示：LDL对内皮细胞的间隙连接通讯功能具有明显的抑制作用，而HDL与维生素C可改善之。LDL致动脉粥样硬化的机理之一可能是抑制了内皮细胞的间隙连接通讯功能，但亦有可能这仅是内皮细胞对损伤的一种代偿反应。而HDL及维生素C对内皮细胞的保护作用可能是通过改善间隙连接的通讯功能来实现的。
The effects of HDL, LDL and vitamins on the communication function of cultured human vascular endothelial cell gap junction were observed by laser scanning confocal microscope and fluorescence after bleaching. Results: Functional gap junctions could be formed between human vascular endothelial cells under culture conditions. The fluorescence response rates of LDL group were 6.691 ± 2.634, 4.153 ± 2.125 and 2.441 ± 0.720, respectively, which were significantly different from those of the control group (P <0.05) There was no significant difference in the fluorescence recovery rate between the vitamin C group and the control group; the fluorescence response rates of the LDL + HDL group were 3.500 ± 0.890, 4.339 ± 1.126 and 5.243 ± 1.278 respectively, (P <0.05). The fluorescence response rates of LDL + vitamin C group were 3.959 ± 1.088, 4.974 ± 1.194 and 8.673 ± 2, respectively. 488, compared with the control group and pairwise comparison, there was a significant difference (P <0.05). The above results suggest that: LDL on endothelial cell gap junctional communication has a significant inhibitory effect, while HDL and vitamin C can be improved. One of the mechanisms of LDL-induced atherosclerosis may be to inhibit the gap junctional intercellular communication of endothelial cells, but it is also possible that this is only a compensatory response of endothelial cells to injury. The protective effect of HDL and vitamin C on endothelial cells may be achieved by improving the communication function of gap junction.