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目的:测定兔口服阿魏酸及口服阿魏酸加生化汤后阿魏酸的血药浓度,并进行药动学比较。方法:采用高效液相色谱法(HPLC)以甲醇水(0.3%冰醋酸)(40∶60)为流动相,色谱柱为Zorbax SBC18(150 mm×4.6 mm,5μm),紫外检测波长323 nm。结果:两组的血药-时曲线均表现为单峰现象,属开放性一室模型。兔口服阿魏酸及兔口服阿魏酸加生化汤后的主要药动学参数如下:Ka(min-1)=0.2061和0.3523,Ke(min-1 )= 1.013×10-2 和5.440×10-3,t1/2Ka (min)= 3.363和1.968,t1/2Ke (min)= 68.41和127.4,Cmax(μg·L-1)=63.41和112.7,AUC(μg·L-1·min)=7312 和2.212×104,CL/F(s)(mL·min-1 )=1.360×10-4 和3.420×10-4,V/F(c)(μg·L-1)=2.493×10-2和3.374×10-2。结论:生化汤对阿魏酸的体内吸收、分布、代谢均产生影响。
Objective: To determine the blood concentration of ferulic acid after oral administration of ferulic acid and oral ferulic acid plus biochemical decoction in rabbits, and to compare the pharmacokinetics. Methods: The mobile phase consisted of methanol water (0.3% glacial acetic acid) (40∶60) as the mobile phase by HPLC. The chromatographic column was Zorbax SBC18 (150 mm×4.6 mm, 5 μm), and the UV detection wavelength was 323 nm. Results: The blood-drug-time curves of the two groups showed a single peak phenomenon and belonged to the open one-compartment model. The main pharmacokinetic parameters of rabbits after oral administration of ferulic acid and rabbit oral ferulic acid plus biochemical decoction were as follows: Ka(min-1)=0.2061 and 0.3523, Ke(min-1)=1.013×10-2 and 5.440×10 -3, t1/2Ka (min) = 3.363 and 1.968, t1/2Ke (min) = 68.41 and 127.4, Cmax (μg·L-1) = 63.41 and 112.7, AUC (μg·L-1 · min) = 7312 And 2.212×104, CL/F(s)(mL·min-1)=1.360×10-4 and 3.420×10-4, V/F(c)(μg·L-1)=2.493×10-2 And 3.374×10-2. Conclusion: Biochemical decoction has effects on the absorption, distribution and metabolism of ferulic acid in vivo.