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目的了解河南省人感染HBV的基因型、基因亚型及血清型的分布。方法采用巢式PCR法对44例HBV感染者血样进行S基因扩增并测序,应用Neighbor-Joining构建系统进化树,分析其基因型和基因亚型;应用Magnius和Norder法进行血清型分析。结果 44例HBV感染者中检出C基因型36例(81.8%),B型8例(18.2%)。B基因型全部为B2亚型,adw2血清型。C基因型中有33例(占75.0%)为C2亚型,adrq+血清型占97.2%(35/36),adrq-血清型占2.8%(1/36)。结论河南省人感染HBV以C基因型为主,其次是B基因型;C基因型中以C2亚型和adrq+血清型为主,B基因型中以B2亚型和adw2血清型为主。
Objective To understand the distribution of HBV genotypes, genotypes and serotypes in Henan Province. Methods The nested PCR was used to amplify and sequence the S gene of 44 HBV infected patients. The phylogenetic tree was constructed using Neighbor-Joining, and the genotypes and genotypes were analyzed. The serotypes were analyzed by Magnius and Norder methods. Results Among the 44 HBV infected patients, 36 C (81.8%) were genotype C and 8 (18.2%) were B genotype. B genotypes are all B2 subtype, adw2 serotype. 33 (75.0%) of the C genotypes were C2 subtypes, with adrq + serotypes accounting for 97.2% (35/36) and adrq- serotypes accounting for 2.8% (1/36). Conclusion HBV genotypes are predominant in Henan Province, followed by genotype B; genotype C is predominantly genotype C2 and adrq + serotypes, while genotype B2 is predominant in genotype B2 and adw2 serotypes.