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对前期获得的转耐盐碱基因GsGST14的大豆株系HF55-GST14-1、HF55-GST14-2、HF55-GST14-3、HF55-GST14-4、HF55-GST14-5的T3代群体进行抽样PCR阳性检测,结果后代群体中转基因阳性个体比例高于80%,说明对这些后代群体的调查能够反映出GsGST14基因对转基因大豆农艺性状的影响。因此对这5个株系的T3代群体农艺性状进行调查及统计学分析。结果表明,5个转基因株系与对照在单株荚数、单株粒数、百粒重、生育期、结荚习性、花色、叶形和蛋白质含量上无显著差异,转基因株系HF55-GST14-1、HF55-GST14-3与HF55-GST14-4的株高显著低于对照。油分含量HF55-GST14-1显著高于对照,HF55-GST14-2显著低于对照。因此,5个转GsGST14基因大豆株系并未在农艺性状上产生较大的不良变异,具有良好的应用前景。
The T3 generation of the soybean line HF55-GST14-1, HF55-GST14-2, HF55-GST14-3, HF55-GST14-4, HF55-GST14-5, which had been transformed with the salinity-tolerance gene GsGST14, was sampled by PCR Positive results showed that the proportion of transgenic individuals in the progeny population was higher than 80%, indicating that the investigation of these progeny population could reflect the influence of GsGST14 gene on the agronomic traits of transgenic soybean. Therefore, we investigated and statistically analyzed the agronomic traits of T3 generation in these 5 lines. The results showed that there was no significant difference in pods, grains per plant, grain weight per plant, growth period, pod habit, flower color, leaf shape and protein content between the five transgenic lines and the control. The transgenic lines HF55-GST14 -1, HF55-GST14-3 and HF55-GST14-4 plants were significantly lower than the control. Oil content HF55-GST14-1 was significantly higher than the control, HF55-GST14-2 was significantly lower than the control. Therefore, the five GsGST14 transgenic soybean lines did not produce large adverse variation in agronomic traits, which has a good application prospect.