论文部分内容阅读
为了分析韩国栗疫病的抗病品种和感病品种的遗传变异和抗病性的筛选,利用抗病性的快速检测法和RAPD(random amplified polymorphic DNA)方法对13个栗树品种进行了抗病性检测和RAPD标记分析。抗病性的快速检测选出了5个抗病品种、5个感病品种和3个中度抗病(或中度感病)品种,并且这一结果与该品种的田间表现相一致。利用筛选的12个随机引物,扩增了100个多态性RAPD片段,但未发现与抗病性或感病性相关的特异RAPD片段。聚类分析结果表明,12个品种大致分为抗病、感病和中度抗病(或中度感病)等3个大组,并与抗病性的快速检测结果基本一致。抗病品种“MANSEKI”表现出了相对于12个品种较远的亲缘关系。
In order to analyze the genetic variation and disease resistance of resistant varieties and susceptible varieties of chestnut blight in Korea, 13 chestnut varieties were tested for disease resistance by rapid detection of disease resistance and random amplified polymorphic DNA (RAPD) Sex testing and RAPD marker analysis. Five resistant varieties, five susceptible varieties and three moderately resistant (or moderately susceptible) cultivars were selected for rapid testing of disease resistance and the results were consistent with the field performance of the cultivar. With the 12 random primers screened, 100 polymorphic RAPD fragments were amplified, but no specific RAPD fragments related to disease resistance or susceptibility were found. Cluster analysis showed that the 12 cultivars could be divided into three groups: disease resistance, susceptibility and moderate disease resistance (or moderate susceptibility), which were basically consistent with the rapid test results of disease resistance. Disease-resistant variety “MANSEKI” showed relatives relative to 12 varieties.