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目的研究骨髓间质干细胞(MSCs)对B淋巴细胞免疫调节的影响。方法体外分离培养骨髓MSCs,流式细胞仪分选出B细胞,共培养,CpG+CD40L刺激48 h后,收集B细胞,再与分选的CD4+CD25-T细胞共培养,流式细胞术检测B细胞对T细胞炎性因子分泌功能的影响;B淋巴细胞与MSCs共培养,CpG+CD40L刺激72 h后,检测B细胞胞内抑炎因子IL-10的表达;分别加入不同调控通路的抑制剂,观察其对MSCs诱导B细胞产生IL-10的影响。结果与MSCs共培养后,B细胞对T细胞炎性因子IFN-γ分泌的抑制作用增强,IL-10表达水平显著增高;加入COX/PGE2信号通路的抑制剂Indomethacin预处理MSCs后,与其共培养的B细胞表达IL-10显著减少。结论 MSCs能诱导高表达IL-10的调节性B淋巴细胞的产生,该机制可能与COX/PGE2信号通路有关。
Objective To study the effect of bone marrow mesenchymal stem cells (MSCs) on immune regulation of B lymphocytes. Methods BMSCs were isolated and cultured in vitro. B cells were sorted out by flow cytometry. The cells were co-cultured and stimulated with CpG + CD40L for 48 h. B cells were collected and co-cultured with sorted CD4 + CD25-T cells. Flow cytometry The effect of B lymphocytes on the secretion of inflammatory cytokines of T cells was examined. B lymphocytes were co-cultured with MSCs. The expression of IL-10 was measured 72 hours after stimulation with CpG + CD40L. Inhibitor, to observe its effects on BMSCs-induced IL-10 production. Results After co-cultured with MSCs, the inhibitory effect of B cells on the secretion of T-lymphocyte inflammatory factor IFN-γ was enhanced and the expression of IL-10 was significantly increased. After pretreatment with Indomethacin, a COX / PGE2 inhibitor, MSCs were co-cultured The B cell expression of IL-10 was significantly reduced. Conclusion MSCs can induce the production of regulatory B lymphocytes with high expression of IL-10, which may be related to the COX / PGE2 signaling pathway.