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应用垂直平板聚丙烯酰胺凝胶电泳技术(PAGE),研究分析了取自浙江舟山朱 家尖养殖区的三疣梭子蟹5种组织中10种同工酶以及肌浆蛋白的表达情况,并进行 生化遗传分析．结果显示,在对40只梭子蟹所检测出的10种同工酶的25个基因座 位中,ADH、ACP-1、CAT-2、EST-2、FDH-2和SOD共6个基因座位为多态,其多态座 位比例(P)为24％,平均杂合度期望值(H)为0．106 6．同时在CAT、LDH、ACP、FDH 4种酶及肌浆蛋白(Pm)中表现出显著的组织特异性．
The vertical plate polyacrylamide gel electrophoresis (PAGE) was used to analyze the expression of ten isoenzymes and sarcoplasmic proteins in five tissues of Portunus trituberculatus collected from Zhujiajian culture area in Zhoushan, Zhejiang Province. The biochemical Genetic analysis. The results showed that there were 6 loci in ADH, ACP-1, CAT-2, EST-2, FDH-2 and SOD in 25 loci of 10 isozymes detected by 40 crabs The polymorphic locus ratio (P) was 24% and the expected average heterozygosity (H) was 0.106 6. At the same time CAT, LDH, ACP, FDH 4 enzymes and sarcoplasmic protein (Pm) showed significant tissue specificity.