论文部分内容阅读
目的:为提高药物疗效,减少用药量,研究复方宁喘雾化剂纯化工艺。方法:分别采用化学方法与药效学方法来考察不同的纯化工艺。用过滤,离心及50%、60%、70%醇化5种方法对水煎药液进行纯化。①化学考察:以处方中主要药物麻黄与黄芩中的麻黄碱,伪麻黄碱及黄芩苷为指标,用HPLC法测定并比较指标含量。②药效学考察:分别观察全白豚鼠经5种纯化药液给药后各治疗组(分别为中药雾化1组、中药雾化2组、中药雾化3组、中药雾化4组、中药雾化5组)、模型组、正常组及西药组的肺与支气管组织形态,肺脏器指数。结果:当药液的醇沉浓度达到60%时,麻黄中的麻黄碱与伪麻黄碱总和为3.04 mg/g,黄芩中黄芩苷含量为77.6 mg/g,分别高于其他方法。药效观察结果显示,中药雾化4组(60%醇化)的治疗效果较好。结论:综合以上含量测定和药效学实验的结果,60%醇化为最佳纯化工艺。
Objective: In order to improve the curative effect and reduce the dosage, the purification process of compound Ningchuan nebulizer was studied. Methods: The chemical methods and pharmacodynamics methods were used to investigate different purification processes. The decoction was purified by filtration, centrifugation and 50%, 60% and 70% alcoholysis methods. ①Chemical examination: Using ephedrine, pseudoephedrine and baicalin, the main prescription drugs in Ephedra and Scutellariae, as indicators, the contents of indicators were determined and compared by HPLC. (2) Pharmacodynamics study: The whole guinea pigs were observed after treatment with 5 kinds of purified liquid drugs (respectively, 1 group of Chinese medicine atomization, 2 groups of Chinese medicine atomization, 3 groups of Chinese medicine atomization, 4 groups of Chinese medicine atomization, Chinese medicine atomized 5 groups), model group, normal group and western medicine group lung and bronchial tissue morphology, lung index. Results: When the alcohol concentration of the solution reached 60%, the sum of ephedrine and pseudoephedrine in ephedra was 3.04 mg / g, and the content of baicalin in skullcap was 77.6 mg / g, which were higher than other methods. Efficacy observations show that the traditional Chinese medicine atomization group 4 (60% alcohol) treatment is better. Conclusion: Based on the above content determination and pharmacodynamics experiment results, 60% alcohol is the best purification technology.