目的:采用RP-HPLC(反相液相色谱法)测定土茯苓胶囊中盐酸小檗碱的含量。方法:色谱柱Kromasil C18柱(4.6 mm×250 mm,5μm);流动相乙腈-磷酸盐缓冲液[0.43%磷酸二氢钠和0.17%十二烷基磺酸钠,并用三乙胺调pH至7.5](47∶53);流速1.0 mL·min-1;检测波长265 nm。结果:盐酸小檗碱在0.042 2～0.527 5μg线性关系良好(r=0.999 1),平均回收率为101.35%,RSD为1.06%。结论:该法快捷、简便、准确,能更好的控制其质量。 Objective: To determine the content of berberine hydrochloride in Tu-Ling capsule by RP-HPLC (reverse phase liquid chromatography). METHODS: Kromasil C18 column (4.6 mm × 250 mm, 5 μm) was used as the mobile phase. The mobile phase consisted of acetonitrile-phosphate buffer [0.43% sodium dihydrogen phosphate and 0.17% sodium dodecyl sulfate, 7.5] (47:53); flow rate 1.0 mL · min-1; detection wavelength 265 nm. Results: Berberine hydrochloride had a good linearity (r = 0.999 1) at 0.042 2 ~ 0.527 5 μg with an average recovery of 101.35% and a RSD of 1.06%. Conclusion: The method is fast, easy and accurate, and can better control its quality.