论文部分内容阅读
目的探讨Serrate1基因转染对小鼠树突状细胞生物学特性的影响。方法将Serrate1基因体外转染小鼠DCs,经G418筛选后,W estern b lot检测DCsSerrate1的表达,流式细胞仪检测转染后DCs表面共刺激分子的表达,混合淋巴细胞反应观察DCs对T淋巴细胞增殖的影响。结果Serrate1基因转染的树突状细胞Serrate1的表达较未转染组明显增高,转染Serrate1基因的DCs表面CD80、CD86等共刺激分子的表达与对照组比较无明显改变(P>0.05);在混合淋巴细胞反应中,转染DCs对T细胞的增殖有抑制作用,而空载体pcDNA 3.1(+)对照组与未转染组的树突状细胞具有强烈的激发T细胞增殖的能力。结论Serrate1基因转染能在不影响DCs表面共刺激分子表达的情况下显著抑制T细胞活化。
Objective To investigate the effect of Serrate1 gene transfection on the biological characteristics of dendritic cells in mice. Methods Serrate1 gene was transfected into mouse DCs in vitro. After screening by G418, the expression of DCsSerrate1 was detected by Western blot. The expression of costimulatory molecules on DCs was detected by flow cytometry. The mixed lymphocyte reaction was used to observe the effect of DCs on T lymphocytes Effect of cell proliferation. Results The Serrate1 expression of Serrate1 gene transfected dendritic cells was significantly higher than that of untransfected cells. The expressions of costimulatory molecules such as CD80 and CD86 on the surface of DCs transfected with Serrate1 gene had no significant difference compared with the control group (P> 0.05). In the mixed lymphocyte reaction, DCs transfected could inhibit the proliferation of T cells, while the empty vector pcDNA3.1 (+) control group and the untransfected group dendritic cells have a strong ability to stimulate T cell proliferation. Conclusion Serrate1 gene transfection can significantly inhibit T cell activation without affecting the expression of costimulatory molecules on the surface of DCs.