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目的:了解大鼠脑出血后血肿周围组织细胞凋亡与神经元特异性烯醇化酶(NSE)的表达及大鼠神经功能缺损程度的关系。方法:用胶原酶注入到大鼠尾状核的方法制作脑出血模型。将大鼠分为脑出血、假手术组、正常组3组。采用苏木素伊红(HE)染色、NSE免疫组织化学染色及TUNEL分别观察各组在脑出血后第6 h、12 h、24 h、48 h、72 h、5 d、7 d时血肿周围NSE及TUNEL的表达。用Longa评分法评价大鼠神经功能缺损程度。结果:大鼠在胶原酶注入6 h后形成稳定的血肿,在造模24～48 h神经功能缺损程度最重;6 h即见到TUNEL阳性细胞的表达,在48 h最明显;NSE从神经元中漏出弥散到细胞间隙也在48 h达高峰。结论:脑出血血肿周围凋亡与神经功能缺损及NSE的变化有关,凋亡可能在脑出血的神经损伤中起重要的作用。
Objective: To investigate the relationship between the apoptosis of perihematoma tissue and the expression of neuron specific enolase (NSE) and the degree of neurological deficits in rats after intracerebral hemorrhage. Methods: The model of intracerebral hemorrhage was made by injecting collagenase into rat caudate nucleus. Rats were divided into intracerebral hemorrhage, sham operation group, normal group 3 groups. The hematoxylin and eosin (HE) staining, NSE immunohistochemical staining and TUNEL were used to observe the changes of the NSE and the expressions of NSE in the hematoma at 6 h, 12 h, 24 h, 48 h, 72 h, 5 d and 7 d after intracerebral hemorrhage TUNEL expression. Longa score was used to evaluate the degree of neurological deficit in rats. RESULTS: After 6 h of collagenase injection, rats formed a stable hematoma, with the highest degree of neurological deficits between 24 and 48 h after modeling. The expression of TUNEL-positive cells was observed at 6 h and the most obvious at 48 h. Meta leakage to the interstitial cell diffusion reached a peak at 48 h. CONCLUSION: Apoptosis around ICH is related to neurological deficits and changes of NSE. Apoptosis may play an important role in the neurological injury of ICH.