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目的:观察消脂汤对非酒精性脂肪性肝炎(NAFLD)小鼠模型的治疗作用及对肠道主要菌群的影响。方法:采用D12492高脂饲料诱导建立NAFLD小鼠模型,造模成功后分别给予不同剂量药物干预,检测血清肿瘤坏死因子-α(TNF-α),白细胞介素-6(IL-6),内毒素(LPS)及相关生化指标,取肝脏行苏木素-伊红(HE)染色和油红O染色;实时荧光定量PCR(Realtime PCR)检测结肠内容物中普雷沃氏菌属、梭杆菌属、双歧杆菌、乳酸杆菌、大肠埃希菌等主要肠道菌群数量的变化。结果:与正常组比较,模型组血清甘油三酯(TG),总胆固醇(TC),血清丙氨酸氨基转移酶(ALT),天冬氨酸氨基转移酶(AST),空腹血糖(FBG),空腹胰岛素(FINS),胰岛素抵抗指数(HOMA-IR),LPS,TNF-α,IL-6水平明显升高(P<0.05,P<0.01);与模型组比较,治疗组FBG,FINS,HOMA-IR,TC,TG显著降低(P<0.01),非诺贝特组血清ALT,AST无明显降低;与正常组比较,模型组小鼠结肠内梭杆菌属、普雷沃氏菌属、双歧杆菌及乳酸杆菌数量明显减少(P<0.01),大肠埃希菌数量升高(P<0.01);正常组与正消组比较无明显差异;与模型组比较,正消组,消脂汤高、中剂量组梭杆菌属、普雷沃氏菌属、双歧杆菌及乳酸杆菌数量明显升高(P<0.05,P<0.01),大肠埃希菌数量显著减少(P<0.01);消脂汤低剂量组、非诺贝特组与模型组无明显差异。结论:消脂汤能显著改善NAFLD小鼠的胰岛素抵抗,减轻肝脏脂肪蓄积和炎症反应,可能与其调整肠道菌群,促进乳酸杆菌、双歧杆菌等优势菌群数量的增长相关。
Objective: To observe the therapeutic effect of Xiaozhi Decoction on nonalcoholic steatohepatitis (NAFLD) mouse model and its effect on the main intestinal flora. Methods: NAFLD mice were induced by D12492 high-fat diet. After successful modeling, different dosages of drugs were given to the mice to detect the levels of tumor necrosis factor-α (TNF-α), interleukin-6 Toxin (LPS) and related biochemical indicators, the liver was stained with hematoxylin-eosin (HE) and oil red O. The contents of Prevotella, Fusobacterium, Bifidobacterium, Lactobacillus, Escherichia coli and other major intestinal flora changes. Results: Compared with normal group, serum triglyceride (TG), total cholesterol (TC), serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), fasting blood glucose (FBG) FINS, HOMA-IR, LPS, TNF-α and IL-6 levels in the treatment group were significantly higher than those in the model group (P <0.05, P < HOMA-IR, TC and TG were significantly decreased (P <0.01), while serum NO and AST in fenofibrate group were not significantly decreased. Compared with the normal group, the mice in the model group were positive for Fusobacterium, Prevotella, Bifidobacterium and Lactobacillus significantly reduced (P <0.01), the number of Escherichia coli increased (P <0.01); there was no significant difference between normal group and Zhengxiao group; compared with model group, Zhengxiao group, Cellulite The quantities of Fusobacterium, Prevotella, Bifidobacterium and Lactobacillus were significantly increased in the soup group and the medium dose group (P <0.05, P <0.01), and the number of Escherichia coli was significantly decreased (P <0.01). Cellulite soup low-dose group, fenofibrate group and the model group no significant difference. Conclusion: Xiaozhi Decoction can significantly improve insulin resistance and reduce hepatic fat accumulation and inflammation in NAFLD mice, which may be related to the regulation of intestinal flora and the increase of the number of dominant bacteria such as lactobacilli and bifidobacteria.