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花卉试管苗移栽成活率的高低,直接影响到组织培养的生产应用价值,本试验以菊花(Chrysanthemum morifolium)、非洲紫罗兰(Saintpaulia ionantha)、观叶凤梨(Ananas comosus)和康乃馨(Dianthus sp.)等四种花卉为材料。进行研究, 不同移栽基质的对比结果表明:其移栽成活率高低顺次为蛭石,珍珠岩和粗砂。而砻糠灰作用不佳,不同场所不同保湿方法的对比结果,以在温室中,移栽苗上加盖烧杯保湿效果较好。 从叶片气孔开张度和保卫细胞大小调查和电镜扫描观察,移栽后2天四种花卉试管苗的气孔和保卫细胞皆明显缩小,一周后基本稳定。试管苗的叶片表面结构比较光滑,移栽后角质层逐渐加厚,表面比较粗糙。
The survival rate of transplanting of flower test-tube seedlings directly affects the production and application value of tissue culture. In this experiment, Chrysanthemum morifolium, Saintpaulia ionantha, Ananas comosus and Dianthus sp. Four kinds of flowers for the material. The results of comparison of different transplanting matrices showed that the transplanting survival rates were vermiculite, perlite and grit, respectively. However, the effect of chaff ash is not good, different places moisturizing method compared to different results in the greenhouse, transplanting seedlings covered with beakers better moisturizing effect. Stomatal opening and guard cell size investigation and electron microscopic scanning of the leaves showed that the stoma and guard cells of four kinds of flower plantlets were significantly reduced 2 days after transplanting, and basically stabilized after one week. The leaf surface structure of test-tube seedlings is relatively smooth, the stratum corneum gradually thickens after transplanting, and the surface is rough.