论文部分内容阅读
PGF_2α是母羊体内主要的天然黄体溶素,动情期后11～13天子宫开始分泌PGF_2α使黄体退化,而PGE_2可能减弱此作用。本实验测定了母羊动情周期13～16天和孕早期子宫卵巢静脉中PGF_2α和PGE_2的浓度,以观察孕酮对子宫分泌PGF_2α和PGE_2的调节作用。实验用成熟母羊,每天两次检查标准动情期,需选择前一动情周期至少15.5～19天的母羊。动情期后12天,母羊用戊巴比妥钠麻醉,做中下腹部切口,在黄体对侧的子宫卵巢静脉内放置一硅聚乙烯插管。对照组母羊不作进一步处理,其中7只不交配,8只交配。实验组母羊动情期后12天双侧卵巢切除,7只不交配,8只交配。另一实验组母羊动
PGF 2α is the major natural luteinizing hormone in ewes, and the uterus begins to secrete PGF_2α to degrade the corpus luteum from 11 to 13 days after estrus, while PGE_2 may weaken this effect. In this study, we measured the concentrations of PGF_2α and PGE_2 in uterine ovary veins during the first 13-16 days of pregnancy and the first trimester of pregnancy, in order to observe the regulation of progesterone on the secretion of PGF 2α and PGE 2 in the uterus. Experiment with mature ewes, check the standard estrus twice daily, need to select the previous estrous cycle at least 15.5 to 19 days of ewes. Twelve days after estrus, the ewes were anesthetized with sodium pentobarbital, mid-abdomen incision was made, and a silicon polyethylene cannula was placed in the uterine ovary vein opposite the luteal phase. The control group of ewes were not further processed, of which 7 did not mate, 8 mated. Eighteen days after estrus in the experimental group were ovariectomized, 7 were not mated, and 8 mated. Another experimental group of ewes move