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目的 研究垂体腺瘤染色体改变及其与肿瘤临床侵袭性的相关性 ,进一步了解垂体腺瘤生物学行为转变的细胞遗传学基础。方法 将 30例经蝶窦垂体腺瘤切除手术中取得的新鲜垂体腺瘤组织制作单细胞悬液 ,体外直接阻滞法获取染色体 ,结合R显带核型分析和间期荧光原位杂交技术 (inter phaseFISH) ,对染色体变异进行观察、分析。结果 垂体腺瘤染色体变异主要为数目改变 ,涉及除 4、16、Y之外的每一条染色体。侵袭性垂体腺瘤的染色体变异几率高于非侵袭性垂体腺瘤。8、9、12、19号染色体的增加在垂体腺瘤染色体变异中十分常见 ,9、11、19号染色体变异和 8号四体 ,9号四体多见于侵袭性垂体腺瘤。间期FISH验证并补充了常规核型分析的结果。结论 侵袭性垂体腺瘤的染色体变异几率高于非侵袭性垂体腺瘤。 8、12号染色体的改变可能在垂体腺瘤的发生起着重要作用 ,而 9、11和 19号染色体的改变可能与肿瘤侵袭性的增加相关
Objective To study the relationship between chromosomal changes of pituitary adenoma and its clinical invasion and to further understand the cytogenetical basis of the biological behaviors of pituitary adenomas. Methods Thirty fresh pituitary adenomas were obtained from resection of pituitary adenoma of the transsphenoidal plexus. Single cell suspensions were obtained. Chromosomes were obtained by direct block method in vitro. Combined with R - band karyotype analysis and interphase fluorescence in situ hybridization interphaseFISH) to observe and analyze the chromosome variation. Results Chromosome variation of pituitary adenomas mainly changed in number and involved every chromosome except 4, 16, Y. The risk of chromosomal aberrations in invasive pituitary adenomas is higher than in non-invasive pituitary adenomas. Chromosome 8,9,12,19 increased in pituitary adenomas chromosomal variation is very common, 9,11,19 chromosome variation and 8th and 4th, 9th most common in invasive pituitary adenoma. Interphase FISH validates and complements the results of conventional karyotyping. Conclusion The incidence of chromosomal abnormalities in invasive pituitary adenomas is higher than that in non-invasive pituitary adenomas. Changes in Chromosome 8 and Chromosome 12 may play an important role in the development of pituitary adenomas, whereas changes in chromosomes 9, 11 and 19 may be associated with increased tumor invasion