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本文提出了应用亚甲蓝为显色试剂的比色测定法。在磷酸盐缓冲液(pH6.8)中反应,生成的络合物经氯仿提取后,分别于一定的波长进行比色测定。方法:取相当于0.1～1.6毫克的样品溶液(样品溶于磷酸盐缓冲液中),置分液漏斗中,加磷酸盐缓冲液(27.6克磷酸二氢钠及71.6克磷酸氢二钠溶于2000毫升水中)至25毫升。加亚甲蓝试液(1克亚甲蓝溶于100毫升水中)1毫升及氯仿10毫升,提取振摇30秒钟,氯仿提取液移入100毫升容量瓶中,水层继续用氯仿提取4次,每次10毫升,氯仿液并入容量瓶中并以氯仿稀释至刻度。于波长635毫微米(青霉素G钠盐及间胺黄),
This paper presents a colorimetric assay using methylene blue as a chromogenic reagent. The reaction was carried out in phosphate buffer solution (pH 6.8), and the resulting complex was extracted with chloroform and subjected to colorimetric determination at a certain wavelength. METHODS: A sample solution equivalent to 0.1-1.6 mg (sample dissolved in phosphate buffer) was placed in a separatory funnel. Phosphate buffer (27.6 g sodium dihydrogen phosphate and 71.6 g sodium phosphate dibasic) was dissolved in 2000 ml water) to 25 ml. Add 1 ml of methylene blue solution (1 g of methylene blue dissolved in 100 ml of water) and 10 ml of chloroform, extract and shake for 30 seconds, transfer the chloroform extract into a 100 ml volumetric flask, and continue to extract the aqueous layer four times with chloroform , 10 ml each time, the chloroform solution was combined into a volumetric flask and diluted to the mark with chloroform. At a wavelength of 635 nm (penicillin G sodium salt and m-amine yellow),