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本方法是根据多烯抗真菌抗菌素的抗酵母作用可为胆固醇抵消的原理而建立。将一纸条沾上发酵液并与另一浸有曲古霉素及胆固醇溶液已经吹干的纸条十字状地置于假丝酵母yu 1200(用葡萄糖营养琼脂)的检定平皿上,于37℃培养17小时后观察抗菌区。曲古霉素-胆固醇溶液是由三体积曲古霉素溶液(1毫克/5毫升丙酮∶水=1∶1)及一体积胆固醇溶液(1毫克/毫升甲醇∶丙酮=9∶1)而配成。多烯抗菌素、非多烯抗菌素、对多烯抗菌素有协同作用的物
This method is based on the principle that the anti-yeast effect of polyene antifungal antibiotics can be offset by cholesterol. A piece of paper stained with a fermentation broth and another immersed with a smear of turamycin and cholesterol solution has been cross-shaped placed on Candida yu 1200 (with glucose nutrition agar) assay plate, at 37 Antibacterial zone was observed after culturing for 17 hours. The curcumin-cholesterol solution was prepared by mixing three volumes of the solution of curcimin (1 mg / 5 ml acetone: water = 1: 1) and one volume of cholesterol solution (1 mg / ml methanol: acetone = 9: 1) to make. Polyene antibiotics, non-polyene antibiotics, polyene antibacterial synergistic effect