Artificial facial nerve prostheses are thought to restore eye-closed function in peripheral facial paralysis patients.At present,however,there is no adequate quantitative or qualitative information regarding myoelectric signal(MES)features for healthy orbiculads oculi muscle(OOM).The present study analyzed MES features of normal OOM in rabbits during the natural continuous eye-opening(N1)state,natural continuous eye-closing(N2)state,natural blink(N3)state,and evoked eye-closing(E)state according to time domain and frequency domain analysis.Results showed that OOM electrical activities in N1 and N2 states,as well as myoelectric amplitude,were low and stable.Nevertheless,during N3 and E states,OOM electrical activities were significantly increased and amplitude was much higher in the E state than in the N3 state.In the time domain,differences in MES peak absolute potential were not significant between N1 and N2 states,in the frequency domain,differences in power spectral density peak frequency of electromyogram signals were significant between two sets of four OOM movement states.These results suggest that OOM significantly contracts and induces eyelid-closing action.In addition,OOM is diastolic during the N1state.A N2 state does not require continuous intensive OOM contraction.Moreover,distinctions of quantitative information in time and frequency domain features of MES can be used as an OOM reference to identify muscle movement patts.