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目的应用再测序芯片技术,调查北京地区社区获得性肺炎中呼吸道病毒的流行情况,探索流行规律,为临床治疗和防治措施制定提供依据。方法采集北京地区社区获得性肺炎患者的鼻咽抽吸物样本,应用再测序芯片技术,筛查流感病毒、冠状病毒、腺病毒、鼻病毒、副流感病毒、呼吸道合胞病毒、偏肺病毒等14种呼吸道病毒,并进行统计分析。结果共采集110份鼻咽抽吸物样本,其中30份样品病毒检测阳性;检出率最高的3种病毒分别是副流感病毒(5.45%)、鼻病毒(4.55%)和腺病毒(4.55%);0岁~4岁儿童组的病毒感染率(61.90%)最高。结论再测序芯片技术通过获得病毒基因序列信息,在进行病毒检测的同时还可以对病毒进行分型。应用该技术了解北京市社区获得性肺炎的病毒病原谱组成和流行规律,为制定防控措施和进一步研究提供科学依据。
Objective To investigate the prevalence of respiratory virus in community-acquired pneumonia in Beijing and explore the epidemic rules by using the technology of re-sequencing chips to provide the basis for clinical treatment and prevention and treatment measures. Methods Samples of nasopharyngeal aspirates from patients with community-acquired pneumonia in Beijing were collected and screened for influenza virus, coronavirus, adenovirus, rhinovirus, parainfluenza virus, respiratory syncytial virus, metapneumovirus 14 kinds of respiratory viruses, and statistical analysis. Results A total of 110 samples of nasopharyngeal aspirate were collected, of which 30 samples were positive for virus. The three most common viruses were parainfluenza virus (5.45%), rhinovirus (4.55%) and adenovirus (4.55% ). The prevalence of virus infection (61.90%) in children aged 0-4 years was the highest. Conclusion Re-sequencing chip technology by obtaining the sequence information of the virus, virus detection at the same time also be able to type the virus. Using this technology to understand the composition and epidemic pattern of viral pathogenic spectrum in community-acquired pneumonia in Beijing and provide scientific evidence for the development of prevention and control measures and further research.