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目的建立测定白花丹药材中β-谷甾醇含量的方法,并比较白花丹药材不同药用部位、不同产地和不同采收期中β-谷甾醇的含量。方法采用高效液相色谱-蒸发光散射检测法,色谱柱为Kromasil C18柱(250mm×4.6mm,5μm),流动相为甲醇,流速为1.0mL·min-1,柱温为30℃,漂移管温度为40℃,载气(N2)压力3.5bar。结果β-谷甾醇1.080～4.860μg与峰面积的自然对数呈良好的线性关系(r=0.9995),平均回收率为99.80%。白花丹根、茎中β-谷甾醇的含量分别为0.2074、0.4064mg·g-1,叶中未测到;测得广西产白花丹中的含量普遍低于云南所产,西双版纳南药园的白花丹中β-谷甾醇含量最高;不同采收期白花丹茎中β-谷甾醇的含量较高。结论所建方法简便、准确、重复性好,可以作为控制白花丹药材质量的方法之一。
OBJECTIVE To establish a method for the determination of β-sitosterol in Chinese white Huadan herbs and compare the content of β-sitosterol in different medicinal parts, different producing areas and different harvesting times of Chinese white Huadan herbs. Methods The column was Kromasil C18 (250 mm × 4.6 mm, 5 μm) with methanol as the mobile phase at a flow rate of 1.0 mL · min-1 and a column temperature of 30 ° C. The drift tube The temperature was 40 ° C and the carrier gas (N2) pressure was 3.5 bar. Results The natural logarithm of β-sitosterol 1.080-4.860μg showed a good linear relationship with the peak area (r = 0.9995). The average recovery was 99.80%. The content of β-sitosterol in the stems was 0.2074 and 0.4064 mg · g-1, respectively, and was not detected in the leaves. The contents of β-sitosterol in the stems of the plants were generally lower than those of Yunnan, Xishuangbanna Southern Park The content of β-sitosterol was the highest in Pistacia chinensis, and the content of β-sitosterol was higher in the stems of Phellodendron amurense in different harvest time. Conclusion The method is simple, accurate and reproducible. It can be used as one of the methods to control the quality of Chinese medicinal materials.