雏凤精为由鹿茸、鸡胎、羊鞭、淫羊藿、人参、补骨脂、当归、黄芪等20余味中药加工精制而成的口服液,主要适用于气血两虚、腰酸背痛、头晕耳鸣、肾亏滑泄及妇女子宫虚冷、月经不调等。为了考察其组方中有效的化学成分,进行了以下几方面的试验。鉴别实验 1.蛋白质鉴别:取本品5滴滴于滤纸上,滴加5滴茚三酮试液,于100℃烘箱中加热5～10分钟呈紫红色。本反应灵敏度高,重现性好。2.丹皮酚定性鉴别:取本品80ml 进行水蒸汽蒸馏,馏液加120ml 乙醚提取,回收乙醚得结晶,将结晶用丙酮2ml 溶解,作为供试品溶液。另取丹皮酚对照品加丙酮制成含生药5mg/ml 的溶液,作为对照溶液,照薄层层析法试验,吸取上述两种溶液各20μl,分别点于同一硅胶 G 薄层板上,以环已烷:醋酸乙酯(3:1)为展开剂,展开,取出,晾 The young phoenix essence is an oral liquid refined from the processing of 20 odor Chinese herbs such as pilose antler, chicken fetus, sheep whip, epimedium, ginseng, psoralen, angelica, and radix astragali. It is mainly used for qi and blood deficiency, back pain, and back pain. Dizziness, tinnitus, kidney loss, leakage and cold uterine uterus, irregular menstruation and so on. In order to investigate the effective chemical composition of its composition, the following tests were conducted. Identification test 1. Protein identification: Take 5 drops of this product onto filter paper, add 5 drops of ninhydrin test solution, and heat it in an oven at 100°C for 5 to 10 minutes. The reaction has high sensitivity and good reproducibility. 2. Paeonol qualitative identification: Take this product 80ml for steam distillation, distillate add 120ml ether extraction, recovery of ether was crystallized, the crystals were dissolved with acetone 2ml, as the test solution. Another paeonol reference substance was added with acetone to prepare a solution containing crude drug 5 mg/ml. As a control solution, according to a thin layer chromatography test, 20 μl of each of the above two solutions was pipetted on the same silica gel G plate. With cyclohexane: ethyl acetate (3:1) as developing agent, expand, remove and dry