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对高效液相色谱柱切换技术中分析流动相、净化时间、预处理柱的长短、预处理流动相纯度、柱切换时间、检测器波长等对系统峰的影响因素进行了系统地讨论.
The effects of mobile phase, purification time, pretreatment column length, pretreatment mobile phase purity, column switching time and detector wavelength on system peak were systematically discussed in the HPLC column switching technique.