论文部分内容阅读
目的探讨heregulin-β1(HRG-β1)对糖酵解的诱导作用及其诱导的糖酵解在乳腺癌细胞MCF7迁移中的作用。方法用PBS(对照组)或HRG-β1处理MCF7细胞12、24和48h,或HRG-β1+草氨酸盐(oxamate,OX)联用处理MCF7细胞24h,收集培养基测定葡萄糖消耗量和乳酸生成量,收集细胞用Western blot检测乳酸脱氢酶A(lactate dehydrogenase A,LDHA)蛋白的表达。用PBS(对照组)、HRG-β1或HRG-β1+OX联用处理MCF7细胞48h,划痕实验检测伤口愈合率以反映细胞的迁移能力。结果HRG-β1处理MCF7细胞12、24和48h组的葡萄糖消耗量、乳酸生成量和LDHA蛋白水平增加均在24h达最大值,与对照组比较差异有统计学意义(P<0.05);与HRG-β1诱导组比较,HRG-β1+OX联用组的葡萄糖消耗量差异无统计学意义(P>0.05),乳酸生成量降低(P<0.01),LDHA蛋白表达量减少(P<0.05);MCF7细胞划痕48h后,对照组和HRG-β1+OX联用组的伤口愈合率相当(P>0.05),均低于HRG-β1诱导组,差异有统计学意义(P<0.001)。结论 HRG-β1通过上调LDHA诱导糖酵解从而促进乳腺癌细胞MCF7的迁移。
Objective To investigate the effect of heregulin-β1 (HRG-β1) on glycolysis and the role of induced glycolysis in the migration of breast cancer MCF7 cells. Methods MCF7 cells were treated with PBS (control group) or HRG-β1 for 12, 24 and 48 h, or HRG-β1 + oxamate (OX) for 24 h. MCF7 cells were collected for measurement of glucose consumption and lactate production The cells were collected and the expression of lactate dehydrogenase A (LDHA) protein was detected by Western blot. MCF7 cells were treated with PBS (control group), HRG-β1 or HRG-β1 + OX for 48h. Scratch test was used to detect wound healing rate to reflect cell migration ability. Results The glucose consumption, lactic acid production and LDHA protein levels of MCF7 cells treated with HRG-β1 for 12, 24 and 48 h were all up to the maximum at 24 h, which was significantly different from that of the control group (P <0.05) β1 + OX group, there was no significant difference in glucose consumption (P> 0.05), lactate production (P <0.01) and LDHA protein expression decreased (P <0.05). The wound healing rate of control group and HRG-β1 + OX group was significantly higher than that of HRG-β1 group (P <0.001) 48 h after MCF7 cell scratching. Conclusion HRG-β1 can promote the migration of breast cancer cell MCF7 by up-regulating LDHA-induced glycolysis.