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蜀恢527(Oryza sativa)因其一般配合力高、所配组合杂种优势强、衍生恢复系多等优点,被认为是现阶段杂交水稻育种的骨干亲本之一。本研究通过亲本性状遗传规律分析,结合全基因组扫描,拟阐明蜀恢527的遗传组成,确定其产量相关性状的关键基因组区域。系谱产量相关性状分析结果显示,结实率高低,每穗实粒数、每穗总粒数和有效穗的多少可能源自IR24-蜀恢527的遗传途径;而千粒重、单株产量的高低和有效穗的多少则可能源自圭630-R1318-蜀恢527的遗传途径。采用1050个SSR引物对相关亲本进行全基因组扫描,构建了蜀恢527基因组来源图谱。分析发现,所有品种共有(无多态性标记)的区段占62.94%;约有17.53%的区段可能来源于多个亲本(多态性标记不足以区分各亲本);在蜀恢527形成过程中,R1318贡献了13.68%区段,辐36-2贡献了0.53%的区段,IR24贡献了1.32%的区段;同时,蜀恢527基因组内包含了4%的自身特有区段。根据性状遗传途径和基因组来源图谱,初步确定了蜀恢527关键基因组区域。整合文献报道有关产量的QTL定位结果,发现了一些可能解释关键区段影响产量的候选QTL位点。本研究对于骨干亲本选育和超级杂交稻育种具有重要意义。
Oryza sativa is considered as one of the key parents for hybrid rice breeding in this stage because of its high general combining ability, strong combined heterosis and many derived restorer lines. In this study, genetic analysis of parents traits, combined with genome-wide scanning, to clarify the genetic composition of Shuhui 527, to determine the yield-related traits of key genomic regions. The analysis of pedigree yield related traits showed that the seed setting rate, grain number per panicle, total grains per panicle and effective panicle might be derived from the genetic pathway of IR24-Shuhui 527; however, grain weight per plant, yield per plant, and effective The number of ears may be derived from Kyu 630-R1318-Shu Hui 527 genetic pathway. A total of 1050 SSR primers were used to perform genome-wide scanning on the related parents to construct a source map of Shuhui 527 genome. The analysis showed that 62.94% of the total cultivars (polymorphic markers) were shared by all cultivars, 17.53% of the total cultivars were derived from multiple parents (the polymorphic markers were not enough to distinguish the parents), and Shuhui 527 In the process, R1318 contributed 13.68% of the segment, the spokes 36-2 contributed 0.53% of the segment, the IR24 contributed 1.32% of the segment, and the Shuhui 527 genome contained 4% of its own unique segment. According to the traits of genetic traits and genomic source maps, the key genomic regions of Shuhui 527 were initially identified. Consensus literature reports on QTL mapping for yield and several candidate QTL loci that may explain the impact of key zones on yields were found. This study is of great significance to the breeding of backbone parents and super hybrid rice breeding.