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目的探讨ZEB-1在肝细胞癌(HCC)组织中的表达及意义。方法采用免疫组织化学SP法和蛋白质印迹分析法检测ZEB-1在62例HCC组织、48例相应癌旁肝硬化组织和10例正常肝组织中的表达,并分析其与HCC临床病理特征的关系。结果免疫组化结果显示ZEB-1在HCC组织及癌旁肝硬化组织中呈阳性表达,阳性率分别为93.5%(58/62)和83.3(40/48)。蛋白质印迹分析显示ZEB-1蛋白在HCC组织中的表达高于相应癌旁肝硬化组织和正常肝组织(P<0.05)。ZEB-1在HCC组织中的表达与临床分期、肿瘤分化程度、有无发生转移、有无门脉癌栓及有无术后复发等因素相关(P<0.05),与肿瘤直径、肿瘤数目、血清AFP水平等因素无关(P>0.05)。结论 ZEB-1的高表达与肝癌的发生、发展密切相关,并且有可能参与HCC细胞的上皮间质转化而促进肝癌的侵袭和转移。
Objective To investigate the expression and significance of ZEB-1 in hepatocellular carcinoma (HCC). Methods Immunohistochemical SP method and Western blotting were used to detect the expression of ZEB-1 in 62 HCC tissues, 48 corresponding adjacent normal liver tissues and 10 normal liver tissues. The relationship between ZEB-1 expression and clinical and pathological features of HCC . Results The results of immunohistochemistry showed that the expression of ZEB-1 was positive in HCC tissues and adjacent normal liver cirrhosis tissues, the positive rates were 93.5% (58/62) and 83.3 (40/48), respectively. Western blot analysis showed that the expression of ZEB-1 protein in HCC tissues was higher than that in the corresponding paracancerous cirrhotic tissues and normal liver tissues (P <0.05). The expression of ZEB-1 in HCC tissue correlated with clinical stage, tumor differentiation, metastasis, portal vein tumor thrombus and postoperative recurrence (P <0.05), but not with tumor diameter, tumor number, Serum AFP levels and other factors unrelated (P> 0.05). Conclusion The high expression of ZEB-1 is closely related to the occurrence and development of hepatocellular carcinoma, and may be involved in the epithelial-mesenchymal transition of HCC cells to promote the invasion and metastasis of hepatocellular carcinoma.