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目的:通过观察左旋延胡索乙素(L-THP)对长春新碱诱导的神经病理性疼痛大鼠痛阈值及其脊髓腰段TNF-α、IL-1β表达的影响,探讨L-THP对化疗痛的作用及其在脊髓水平的作用机制。方法:根据痛阈值大小筛选出合格的大鼠30只,并随机分为5组:对照组、模型组、L-THP 3mg/kg、6mg/kg、12mg/kg组。采用隔日腹腔注射长春新碱(125μg/kg)的方法建立化疗痛动物模型,采用不同剂量的L-THP治疗化疗痛大鼠,并测定大鼠机械痛阈值和热痛阈值,用ELISA方法检测脊髓腰段TNF-α、IL-1β含量,比较各组的变化。结果:与对照组比较,模型组机械痛阈值和热痛阈值明显降低(P<0.05或P<0.01);与模型组比较,L-THP12mg/kg组和L-THP 6mg/kg组机械痛阈值或热痛阈值显著增高(P<0.05或P<0.01)。另外,模型组TNF-α、IL-1β水平与对照组组比较显著升高(P<0.01),L-THP 12mg/kg组和L-THP 6mg/kg组TNF-α、IL-1β表达较VCR组明显降低(P<0.05或P<0.01)。结论:左旋延胡索乙素能提高化疗痛大鼠的痛阈值,该作用可能与其抑制脊髓腰段TNF-α、IL-1β等炎性细胞因子的表达有关。
OBJECTIVE: To investigate the effect of L-THP on the pain threshold and the expression of TNF-α and IL-1β in spinal cord of rats with vincristine-induced neuropathic pain and to investigate the effect of L-THP on the pain of chemotherapy-induced pain Role and its mechanism of action at spinal level. Methods: Thirty healthy rats were screened according to the threshold of pain. They were randomly divided into 5 groups: control group, model group, L-THP 3mg / kg, 6mg / kg and 12mg / kg group. The animal models of chemotherapy pain were established by intraperitoneal injection of vincristine (125 μg / kg) every other day. The rats were treated with different doses of L-THP, and the mechanical and thermal pain thresholds were determined. The spinal cord was detected by ELISA The levels of TNF-α and IL-1β in lumbar segment were compared. Results: Compared with the control group, the mechanical pain threshold and thermal pain threshold of the model group were significantly decreased (P <0.05 or P <0.01). Compared with the model group, the mechanical pain threshold of the L-THP 12 mg / kg group and L-THP 6 mg / Or pain threshold was significantly higher (P <0.05 or P <0.01). In addition, the levels of TNF-α and IL-1β in model group were significantly higher than those in control group (P <0.01). The expression of TNF-α and IL-1β in L-THP 12 mg / kg and L-THP 6 mg / VCR group was significantly lower (P <0.05 or P <0.01). CONCLUSION: L-Tetrahydropalmatine can increase the pain threshold in rats with chemotherapy, which may be related to the inhibition of inflammatory cytokines such as TNF-α and IL-1β in spinal cord.