论文部分内容阅读
以狗枣猕猴桃试管苗的叶片为外植体,接种于含3%蔗糖和0.2%Gelrite的BW培养基上,外加2,4-D(0,0.1,1和10μmol/L)与玉米素(0,1和10μmol/L)的12种激素组合,置于25℃,光周期为16/8h,光照强度为4000lx的条件下培养。在含1或10μmol/L2,4-D与1或10μmol/L玉米素组合的BW培养基上,产生了体细胞胚,并分化出小植株。随着玉米素浓度的增加,每个外植体上的胚再生频率和体细胞胚的数量也随之增加。同时以叶片为外植体产生的狗枣猕猴桃试管苗的愈伤组织表层产生了不定芽,并抽长成枝。发枝率随着玉米素浓度的增加而增加,并受高浓度的2,4-D所抑制。枝芽转接到含1μmol/LNAA的BW培养基上生根,长成小植株。
The leaves of the kiwifruit in vitro were inoculated on BW medium containing 3% sucrose and 0.2% Gelrite, supplemented with 2,4-D (0,0.1,1 and 10 μmol / L) and zeatin 0, 1 and 10 μmol / L) were cultured under the conditions of 25 ℃, photoperiod of 16 / 8h, light intensity of 4000lx. Somatic embryos were generated on BW medium containing 1 or 10 μmol / L 2,4-D in combination with 1 or 10 μmol / L zeatin and plantlets were differentiated. As the concentration of zeatin increased, the frequency of embryo regeneration and the number of somatic embryos on each explant also increased. In the meantime, adventitious buds were formed on the callus surface of the kiwifruit test-tube seedlings produced by the leaves as explants, and the shoots were long branches. The rate of shoot growth increased with increasing zeatin concentration and was inhibited by high 2,4-D concentrations. The shoots were transferred to BW medium containing 1μmol / L NAA for rooting and growing into small plants.