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目的:对湿生扁蕾乙醇提取物进行血清药物化学研究,探讨湿生扁蕾乙醇提取物的药效物质基础。方法:通过高效液相色谱法(HPLC)在相同的色谱条件下分别测定湿生扁蕾乙醇提取物、空白血清和不同灌胃剂量的给药血清的HPLC图谱,通过比较各成分的相对保留时间,确定湿生扁蕾乙醇提取物大鼠灌胃后血中移行成分及不同给药剂量湿生扁蕾含药血清中血中移行成分的差异。结果:随着给药剂量的增加,湿生扁蕾含药血清中的血中移行成分及其含量也相应增加。在湿生扁蕾给药剂量为5.16g/kg时,给药血清中发现7种移行成分,其中4种为原型成分,且检出3种分别为当药黄素,当药醇苷和木犀草素,另外3种可能为原型成分的次生代谢产物或新生代谢产物。结论:给药剂量会对湿生扁蕾的血中移行成分的种类和含量有较大影响,7种血中移行成分可能为湿生扁蕾的药效成分,但是有些成分含量比较低,是否能成为有效成分有待继续考究,对其进行进一步研究有助于阐明湿生扁蕾的药效物质基础和作用机理。
OBJECTIVE: To study the serum drug chemistry of the ethanol extract from Hyphomycetes angustifolia and to explore the pharmacodynamic basis of the ethanol extract of Hyla sibirica. Methods: The HPLC profiles of ethanol extract, blank serum and different dosages were determined by high performance liquid chromatography (HPLC) under the same chromatographic conditions. The relative retention time of each component , To determine the composition of wet placenta ethanol extract rats after intragastric administration of blood components and different doses of wet Xiaoyanlei serum containing blood migration components. Results: With the increase of the dosage, the content and the content of blood translocation in the drug-containing serum of the hyphomycetes were correspondingly increased. When the dosage of hygienic buds was 5.16g / kg, seven kinds of migratory components were found in the serum of the administration, of which four were the prototype components and three were detected as the flavonoids, Hypocrellin, the other three may be secondary components of the prototype metabolites or new metabolites. CONCLUSION: The dosage of Daphniphyllum ointment has a significant effect on the type and content of the migrating components in the blood of Hyphomycetes Sinensis. The seven kinds of blood migrating components may be the active ingredient of hygrophytes, but some of them are relatively low in content or not To be an active ingredient to be further studied, to further study it helps to clarify the pharmacological effects of wet flat buds the material basis and mechanism.