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诱导水稻幼穗愈伤组织的去分化培养基,在含有2.4—D 情况下加入一定量的 KT,对再生植椿有较好的作用。本试验得出:以去分化培养基中2.4—D4毫克/升+KT10毫克/升的分化率比对照提高2倍。低温处理不同品种的愈伤组织,经继代培养30天后于5～6℃条件下处理10天后再转入分化培养基中。两个品种的分化率趋势一致,分别比对照提高3～4倍。愈伤组织经继代培养120天后仍有再生植株能力。
Induction of dedifferentiation medium in young panicle callus, adding a certain amount of KT in the presence of 2.4-D, had a good effect on regenerated plantlets. The experiment results showed that the differentiation rate of 2.4-D4 mg / L + KT10 mg / L in dedifferentiation medium was 2 times higher than that of the control. Cryogenic treatment of different varieties of callus, subculture 30 days after treatment at 5 ~ 6 ℃ conditions for 10 days and then transferred to differentiation medium. The trend of differentiation rate of the two cultivars was the same, which increased by 3 ~ 4 times respectively than the control. Callus still has regenerative plant ability after subculture for 120 days.