论文部分内容阅读
[目的]探讨神经元特异性烯醇化酶(NSE)在肺癌中的表达及其诊断价值。[方法]收集人肺癌、癌周及远癌组织各26份 ,制备和定量总RNA水平 ;并在肺癌组织匀浆后 ,分析组织中NSE的浓度变化 ;同时分析51例原发性肺癌和27例良性肺病患者血清NSE水平 ,以探讨血NSE在肺癌诊断中的临床价值。[结果]肺癌组织NSE表达显著高于癌周及远癌组织(P<0.001) ,并与总RNA浓度呈正相关关系(r=0.40,P<0.01) ;血清NSE在良性肺病为(7.13±3.53)ng/ml,原发性肺癌为(19.20±10.14)ng/ml,前者明显低于后者(P<0.01)。[结论]检测血中NSE非常有助于肺癌的诊断和鉴别诊断。
[Objective] To investigate the expression of neuron-specific enolase (NSE) in lung cancer and its diagnostic value. [Methods] Twenty-six human lung cancer, peritumorous and distant cancer tissues were collected, total RNA levels were prepared and quantified, and the concentration of NSE in tissues was analyzed after lung cancer homogenate. At the same time, 51 cases of primary lung cancer and 27 cases of benign were analyzed. Serum NSE levels in patients with lung disease to explore the clinical value of blood NSE in the diagnosis of lung cancer. [Results] The expression of NSE in lung cancer tissues was significantly higher than that in peripheral and distant cancer tissues (P<0.001), and was positively correlated with the total RNA concentration (r=0.40, P<0.01). The serum NSE in benign lung diseases was (7.13±3.53) ng /ml, primary lung cancer was (19.20±10.14) ng/ml, the former was significantly lower than the latter (P<0.01). [Conclusion] The detection of NSE in blood is very helpful for the diagnosis and differential diagnosis of lung cancer.