研究了温度、pH值、底物流速、底物浓度、底物的葡萄糖当量值 (DE)等因素对分子筛固定化黑曲霉葡萄糖淀粉酶水解淀粉液化液反应性能的影响规律 ,考察了固定化酶催化剂的寿命 .在适宜条件下 ,供给质量百分数 10 %的淀粉液化液 ,连续 2 2d可产生DE值 95以上的糖化液 ,运转 30d ,活力仅下降 2 5 % .以DE值 15的玉米淀粉液化液为底物 ,糖化液中葡萄糖的质量百分数可达 97%以上 . The effects of temperature, pH value, substrate flow rate, substrate concentration and substrate dextrose equivalent (DE) on the reactivity of starch hydrolyzate immobilized with Aspergillus niger glucoamylase were investigated. The effects of immobilization Under the proper conditions, the starch liquefaction solution with the mass percentage of 10% could produce the saccharified liquid with the DE value of 95 or more continuously for 2 days, and the activity decreased only 25% after 30 days of operation.With the corn starch whose DE value is 15 Liquefaction as a substrate, saccharified liquid mass percentage of glucose up to 97%.