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严重急性呼吸综合征(SARS)自2002年11月在中国广东爆发后,已迅速蔓延成为全球性传染疾患。为了了解SARS冠状病毒的特征,对先前SARS冠状病毒PCR检测呈阳性的来自广东的3份尸检肺组织标本、2份尸检脾组织标本,来自北京的2份咽拭子标本和1份血清标本,利用10种不同的细胞系分离病毒。结果显示,上述标本在感染细胞后,分别可在293、Vero-E6、Vero、RD和HeLa细胞系中产生细胞病变(CPE)。不同标本在上述细胞系中致CPE的能力不同,但CPE出现的时间和病变形态学特征无显著性差异。以恢复期SARS病人血清为抗体,用间接免疫荧光法对感染后细胞培养的检测,冠状病毒RT-PCR对感染后细胞RNA的检测,初步证明分离的病毒为冠状病毒。结果再次证明冠状病毒为SARS的病原,它具有较广泛的器官分布和细胞感染能力。血清中SARS冠状病毒的分离,高度提示在SARS发病过程中存在有病毒血症。
Severe acute respiratory syndrome (SARS) has spread rapidly into a global contagious disease since its outbreak in Guangdong, China, in November 2002. To understand the characteristics of the SARS coronavirus, three autopsy lung samples from Guangdong, two autopsy splenic tissue samples, two throat swab specimens and one serum sample from Beijing were obtained from previous SARS-CoV PCR-positive PCR tests. Viruses were isolated using 10 different cell lines. The results showed that these cells could produce cytopathic effect (CPE) in 293, Vero-E6, Vero, RD and HeLa cell lines respectively after infection. The ability of different specimens to induce CPE in these cell lines was different, but there was no significant difference in the appearance time of CPE and morphological features of lesions. Serum samples from patients with convalescent SARS were detected by indirect immunofluorescence (FCM), and the RNA was detected by RT-PCR after infection. The results showed that the isolated virus was coronavirus. The results again proved that the coronavirus is the causative agent of SARS, which has a broader organ distribution and cell infectivity. Separation of SARS coronavirus in serum is highly suggestive of viremia during the pathogenesis of SARS.