论文部分内容阅读
目的观察产科抗磷脂综合征患者血清对脐静脉内皮细胞活化的影响。方法用采集的16例APS患者和10例健康对照者血清分别处理体外培养的脐静脉内皮细胞,用逆转录定量聚合酶链反应检测脐静脉内皮细胞上细胞间粘附分子-1(ICAM-1)和E-选择素(E-selectin)mRNA表达,用流式细胞仪检测二者蛋白的表达。结果APS血清处理后脐静脉内皮细胞上粘附分子ICAM-1和E-selectin mRNA和蛋白均比对照组明显升高,差异有显著性(P<0.05)。结论抗磷脂综合征患者血清可激活脐静脉内皮细胞,使粘附分子表达增加。
Objective To observe the effect of sera from patients with obstetric antiphospholipid syndrome on the activation of umbilical vein endothelial cells. Methods Human umbilical vein endothelial cells (UUHs) were harvested from 16 APS patients and 10 healthy controls respectively. ICAM-1 was detected by reverse transcription quantitative polymerase chain reaction ) And E-selectin mRNA expression were detected by flow cytometry of the two protein expression. Results The mRNA and protein expressions of ICAM-1, E-selectin and ICAM-1 were significantly increased in APS-treated human umbilical vein endothelial cells compared with the control group. The difference was significant (P <0.05). Conclusion Serum of antiphospholipid syndrome activates human umbilical vein endothelial cells and increases the expression of adhesion molecules.