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目的探讨蛋白酶体抑制剂Lactacystin(LAC)及其与顺铂(DDP)联合对宫颈癌HeLa细胞增殖和凋亡的影响。方法采用四甲基偶氮唑蓝(MTT)法和流式细胞技术(FCM)检测宫颈癌HeLa细胞的增殖抑制率和凋亡率。细胞并分为4组:对照组、LAC组、DDP组及LAC+DDP,每组均干预HeLa细胞48h。结果MTT检测结果显示,LAC可有效抑制HeLa细胞的增殖,呈浓度(r=0.864,P<0.05)及时间依赖性(r=0.926,P<0.05),48h时的半数抑制量(IC_(50))为4.98μmol/L;与DDP组比较,LAC+DDP组对细胞增殖的抑制作用明显增强(P<0.05)。DDP的IC_(50)由63.71μmol/L下降至28.93μmol/L。FCM检测结果显示,细胞凋亡率随LAC作用时间的延长而上升,具有时间依赖性(r=0.869,P<0.05);与DDP组凋亡率[(17.49±3.82)%]和LAC组[(24.98±4.61)%]比较,LAC+DDP组[(38.06±5.72)%]诱导细胞凋亡的作用显著增强(P<0.05)。结论蛋白酶体抑制剂LAC可有效抑制宫颈癌HeLa细胞的体外增殖作用,并通过诱导宫颈癌HeLa细胞凋亡,增强DDP对宫颈癌HeLa细胞株的作用。
Objective To investigate the effects of proteasome inhibitor Lactacystin (LAC) and its combination with cisplatin (DDP) on proliferation and apoptosis of cervical cancer HeLa cells. Methods The proliferation inhibition rate and apoptosis rate of cervical cancer HeLa cells were detected by MTT assay and flow cytometry (FCM). The cells were divided into 4 groups: control group, LAC group, DDP group and LAC + DDP, each group interfered HeLa cells 48h. Results MTT assay showed that LAC could effectively inhibit the proliferation of HeLa cells in a concentration-dependent manner (r = 0.864, P <0.05) and in a time-dependent manner (r = 0.926, )) Was 4.98μmol / L. Compared with DDP group, the inhibitory effect of LAC + DDP group on cell proliferation was significantly enhanced (P <0.05). The IC 50 of DDP decreased from 63.71μmol / L to 28.93μmol / L. The results of FCM showed that the apoptotic rate increased with the prolongation of LAC in a time-dependent manner (r = 0.869, P <0.05), compared with that in DDP group (17.49 ± 3.82%) and LAC group (24.98 ± 4.61)%], LAC + DDP group [(38.06 ± 5.72)%] induced apoptosis significantly increased (P <0.05). Conclusion The proteasome inhibitor LAC can effectively inhibit the proliferation of cervical cancer HeLa cells in vitro and induce the apoptosis of cervical cancer HeLa cells and enhance the effect of DDP on HeLa cells.