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目的研究橘核传统药用品种大红袍Citrus reticulata‘Dahongpao’中柠檬苦素类化合物生物合成机制与转运规律。方法利用UPLC法测定橘种子生长发育过程中茎、叶、果皮及种子中的柠檬苦素、诺米林、黄柏酮等柠檬苦素类化合物的量,采用灰色关联度分析方法,与克隆获得的角鲨烯合成酶基因(ss)、角鲨烯环氧酶基因(se)、葡萄糖基转移酶基因(lgt)表达量进行关联度分析。结果不同器官中柠檬苦素类化合物在种子中得到最大积累;不同器官中柠檬苦素类化合物的积累与ss、se、lgt基因表达均有较大的关联,但在种子中柠檬苦素的积累与ss、se、lgt基因表达最密切,其中ss基因表达对柠檬苦素、诺米林、黄柏酮的积累贡献最大。结论本实验为柠檬苦素类化合物合成机制与转运规律提供可靠、科学的依据。
Objective To study the mechanism of biosynthesis and transport of limonoids in Citrus reticulata’Dahongpao ’, a traditional medicinal species of orange. Methods The amount of limonoids, such as limonin, numillin and phellodendron, in stem, leaf, peel and seeds during the growth and development of orange seeds were determined by UPLC. The gray relational grade Squalene synthase gene (ss), squalene epoxidase gene (se), glucosyltransferase gene (lgt) expression levels of relevance analysis. Results Limonoids were found to accumulate the most in different organs. The accumulation of limonoids in different organs was correlated with the expression of ss, se and lgt genes. However, limonin accumulation in seeds And ss, se, lgt gene expression most closely, which ss gene expression on the limonin, numilin, the largest contribution to the accumulation of vinorelbine. Conclusion This experiment provides a reliable and scientific basis for the synthesis mechanism and transport regulation of limonoid compounds.