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以P效率差异显著的IR74(P高效型 )、IR71331(中间型 )及IR71379(P低效型 )等 3个品种为供试材料 ,采用水培法研究了这 3类水稻在低P胁迫下P的吸收、转运及利用效率等P效率差异的原因以及P吸收动力学特征参数、R/S、酸性磷酸酯酶 (acidphosphoesterase ,简称APase)与核糖核酸酶 (ribonuclease,简称RNase)等表现 .结果表明 ,在低P胁迫下 ,P效率的高低是由水稻对P的吸收率、转运率以及利用效率综合作用的结果 ,存在基因型差异 ,P高效基因型IR74和P效率中间型的IR71331具有高的P吸收率 ,而IR71379P的吸收率低 .不同P效率水稻在低P胁迫下 ,其Km、Cmin、Imax、R/S与Apase相对活性等参数表现出基因型的差异 ,小的Km 和Cmin,大的Imax和R/S及高的APase升幅是水稻对P胁迫的生理适应性特征 ,也是植株在低P胁迫下较为敏感生理指标 .其中各类型水稻叶片中RNase活性在低P胁迫下均表现大幅度上升 ,在品种间无显著差异 ,说明该酶是逆境胁迫并非低P胁迫的特征反应 .
Three cultivars, IR74 (P efficient), IR71331 (intermediate) and IR71379 (P inefficient) with significant difference in P efficiency, were used as materials to study the effects of low P stress P absorption, transport and utilization efficiency, P absorption kinetics characteristic parameters, R / S, acid phosphoesterase (APase) and ribonuclease (RNase) etc.Results The results showed that under low P stress, the efficiency of P was the result of the combined effect of P absorption rate, translocation rate and utilization efficiency of rice, and there was a genotype difference. The high efficient genotype IR74 and P efficient intermediate IR71331 had high While the IR71379P had a low absorption rate.The genotypes of the relative activity of Km, Cmin, Imax, R / S and Apase in different P-efficiency rice under low P stress showed the difference in genotypes, and the small Km and Cmin , Large Imax and R / S and high APase increase were the physiological adaptability characteristics of rice under P stress and also the more sensitive physiological indexes of plants under low P stress.The RNase activity of all kinds of rice leaves under low P stress The performance of a substantial increase in variety There was no significant difference between the two enzymes, indicating that the enzyme is stress response is not low-P stress characteristic response.