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【目的】从基因组层次研究草菇低温自溶异常代谢的分子特征。【方法】对21个真菌物种进行全基因组系统发生分析,进而选取其中具有代表性的物种进行比较基因组学分析,系统研究草菇异常代谢分子特征。【结果】全基因组系统发生分析结果显示草菇位于草腐菌所形成簇的底端。基于全基因组系统发生树,由于担子菌和子囊菌属于完全不同的演化路径,所以选取担子菌中具有代表性的9个物种进行比较基因组学分析,结果显示相比于其它草腐菌,草菇基因家族具有一定的收缩趋势。进一步对不同范畴的基因家族数目进行比较,结果显示3个大于200的草菇基因家族(fam1、fam4和fam6)分别发生了显著扩增,且在总数上也显著高于其它物种,表明草菇的这一分子特征与其异常代谢相关。【结论】3个草菇基因家族(>200)显著扩增提示其特定基因家族功能加强,很可能与草菇低温自溶密切相关。
【Objective】 The objective of this study was to study the molecular characteristics of the abiotic metabolism of volvaceous mushrooms at the genome level. 【Method】 The genome-wide phylogenetic analysis of 21 fungal species was carried out, and then the representative species were selected for comparative genomics analysis to systematically study the abnormal metabolic molecular characteristics of straw mushroom. 【Result】 Genome-wide phylogenetic analysis showed that Agrobacterium tumefaciens was located at the bottom of tufts formed by the grass rot fungi. Based on the genome-wide phylogenetic tree, comparative analysis of nine representative species of Basidiomycetes by comparative genomics showed that Basidiomycetes and Ascomycota belonged to completely different evolutionary pathways. Compared with other grass rot fungi, Gene family has a certain tendency to shrink. Further comparison of the number of gene families in different categories showed that three families of genera of greater than 200 genes (fam1, fam4 and fam6) were significantly amplified, respectively, and were significantly higher than the other species in total, indicating that the mushroom This molecular feature is related to its abnormal metabolism. 【Conclusion】 The significant amplification of the three genera of strawberry (> 200) suggests that the function of specific gene families is enhanced, which may be closely related to the low-temperature autolysis of mushroom.