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目的探讨低度恶性肌纤维母细胞肉瘤(low grade myofibroblastic sarcoma,LGMS)组织中NLRP3炎性体蛋白的表达与LGMS发展、转移和预后的关系及其意义。方法采用免疫组织化学法检测13例手术切除的经病理检查确诊的LGMS瘤组织和瘤旁组织中Caspase-1、ASC和NLRP3蛋白的表达,分析瘤组织中3个NLRP3炎性体蛋白表达与临床病理特征的关系,并采用Pearson相关检验检测3个NLRP3炎性体蛋白两两之间的相关性。结果瘤旁组织中Caspase-1、ASC和NLRP3蛋白表达的平均吸光度值分别为(0.40±0.01)、(0.42±0.01)和(0.37±0.02),均明显高于肌纤维母细胞瘤组织,差异有统计学意义(P<0.05);LGMS转移患者和复发患者瘤组织中Caspase-1、ASC和NLRP3蛋白表达明显低于未发生转移患者和原发肿瘤患者(P<0.05);而瘤组织中NLRP3炎性体蛋白表达与年龄、性别和原发部位是否有慢性炎性反应无关(P>0.05)。两组中Caspase-1、ASC和NLRP3蛋白表达水平两两之间均呈明显正相关(P<0.05)。结论 NLRP3炎性体相关蛋白主要在LGMS瘤旁组织中高表达,且其与LGMS转移与预后有关。
Objective To investigate the relationship between the expression of NLRP3 inflammasome and the development, metastasis and prognosis of low-grade myofibroblastic sarcoma (LGMS) and its significance. Methods Immunohistochemistry was used to detect the expression of Caspase-1, ASC and NLRP3 proteins in 13 surgically excised LGMS tissues and adjacent normal tissues. Immunohistochemical staining was used to detect the expression of three NLRP3 inflammasome proteins in the tumor tissue Pathological features of the relationship between Pearson correlation test and three NLRP3 inflammasome protein between two pairs of correlation. Results The average absorbance values of the expressions of Caspase-1, ASC and NLRP3 in tumor tissues were (0.40 ± 0.01), (0.42 ± 0.01) and (0.37 ± 0.02), respectively, which were significantly higher than those in myofibroblastoma (P <0.05). The expressions of Caspase-1, ASC and NLRP3 in LGMS metastasis and recurrence patients were significantly lower than those in patients without metastasis and in primary tumor (P <0.05). However, the expression of NLRP3 Inflammatory protein expression was not related to age, sex and the presence of chronic inflammatory reaction in the primary site (P> 0.05). The expressions of Caspase-1, ASC and NLRP3 in both groups were positively correlated with each other (P <0.05). Conclusion The NLRP3 inflammasome related protein is mainly expressed in the peritumoral tissues of LGMS, and it is related to the metastasis and prognosis of LGMS.