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给雄性Wistar大鼠sc不同剂量的镉金属硫蛋白(CdMT),结果表明镉接触组尿镉、尿钙和尿蛋白浓度都高于对照组,并与镉剂量间存在剂量效应关系,而血清游离钙浓度无变化;镉接触组肾皮质钠泵和钙泵活性低于对照组,体外试验结果也显示镉能抑制钙泵活性,谷胱甘肽(GSH)和半胱氨酸对这种抑制有保护作用}镉接触组肾皮质GSH含量低于对照组,而丙二醛(MDA)含量则高于对照组,高剂量组肾皮质cAMP/cGMP比值低于对照组。肾皮质钙泵活性、cAMP/cGMP比值、GSH含量三者都与尿钙浓度间呈负相关,MDA含量则与尿钙呈正相关,揭示镉引起的钙尿症是由肾重吸收钙障碍造成的,可能与镉引起的肾脏钠泵、钙泵、环核苷酸、GSH和脂质过氧化等改变有关。
Different doses of cadmium metallothionein (CdMT) were administered to male Wistar rats. The results showed that the concentrations of urinary cadmium, urinary calcium and urinary protein in cadmium exposure group were higher than those in the control group, and there was a dose-response relationship between cadmium dose and serum free Calcium concentration did not change; renal cortical sodium pump and calcium pump activity in cadmium exposure group was lower than the control group, and in vitro test results also showed that cadmium can inhibit calcium pump activity, glutathione (GSH) and cysteine on this inhibition Protection} The level of GSH in renal cortex of cadmium exposed group was lower than that of control group, while the content of malondialdehyde (MDA) in cadmium exposure group was higher than that in control group. The cAMP / cGMP ratio in renal cortex of high dose group was lower than that of control group. The activity of cortical calcium pump, cAMP / cGMP ratio, GSH content were negatively correlated with urinary calcium concentration, and MDA content was positively correlated with urinary calcium, revealing that cadmium-induced calcineuria was caused by kidney reabsorption of calcium disorders , May be related to cadmium-induced renal sodium pump, calcium pump, cyclic nucleotide, GSH and lipid peroxidation and other changes.