精氨酸脱亚胺酶,在精氨酸水解途径中是精氨酸转变为瓜氨酸的催化酶。由于该酶的性质不稳定,故纯化时应快速,并于4℃下操作。此外,该酶还对蛋白酶和金属离子尤其是锌离子敏感,接触后即失活。所以在细胞超声粉碎前,所有含EDTA缓冲液的细胞悬液中需加上一种半胱氨酸酶的抑制物、转-环氧琥珀酰-L-亮氨酰胺-丁烷(E64),这是维持贾 Arginine deiminase is a catalytic enzyme that converts arginine to citrulline in the arginine hydrolysis pathway. Due to the unstable nature of the enzyme, purification should be rapid and at 4 ° C. In addition, the enzyme is also sensitive to proteases and metal ions, especially zinc ions, and is deactivated upon contact. Therefore, before cell sonicating, a cell lysate containing EDTA buffer was added with a cysteine ​​enzyme inhibitor, trans-epoxysuccinyl-L-leucinamide-butane (E64) This is to maintain Jia