芸苔素内酯(1)是Grove等人1979年首次报道的一种固醇类强效植物生长调节剂,它及相关类固醇在化学、生物学及农田中的应用引起了广泛的关注(Cutler等,1991;Khripach等,1999;SaKutar等,1999)。近期芸苔素内酯的代谢研究,特别是24-表-芸苔素内酯的代谢研究表明:在各类完整植物和培养细胞中,代谢路径通常是首先在C-2,C-3和C-23的羟基上葡萄糖化,然后在C-25、C-26位置上酶催化羟基化形成化合物(2)和(3)(图1),如:Hai等于 Brassinolide (1) is the steroid potent plant growth regulator first reported by Grove et al. In 1979, and its use in chemistry, biology and farmland has attracted much attention (Cutler Et al., 1991; Khripach et al., 1999; Sa Kutar et al., 1999). Recent metabolic studies of brassinolide, in particular the metabolism of 24-epi-brassinolide, have shown that in all types of intact plants and cultured cells, metabolic pathways are usually the first in the C-2, C-3 and C-23, followed by enzymatic hydroxylation at C-25, C-26 to form compounds (2) and (3) (Figure 1)