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作者在实验室条件下成功地建立了酶联免疫吸附试验(ELISA)测算白纹伊蚊吸小鼠血血量的方法。吸血方法是将小鼠置于蚊笼中让羽化后一直喂以糖水的白纹伊蚊叮咬吸血,吸血前停饲糖水饥饿6小时以上,部分蚊虫只让其停落在鼠体上1分钟,作为小吸血量的样本。ELISA试验方法是,在直径为4.2mm的小铁珠外,涂布聚碳酸盐塑料;小珠用0.1M的碳酸钠缓冲液(pH9.6)作1:10~4稀释的羊抗鼠IgG抗血清,在4℃下致敏过夜,用含0.05%吐温的PBS—吐温洗涤3次;每个致敏的小珠置于微量反应板的1
The authors successfully established in laboratory conditions enzyme-linked immunosorbent assay (ELISA) measured Aedes albopictus mosquito blood in mice. Vampire method is to place the mice in the mosquito cage so that feathers have been fed with sugar water of Aedes albopictus bites sucking blood before stopping bleeding sugar water hungry more than 6 hours, some mosquitoes only allowed to stop on the mouse for 1 minute, As a small amount of blood sucking samples. ELISA test method is, outside the small diameter of 4.2mm iron beads coated polycarbonate plastic; beads with 0.1M sodium carbonate buffer (pH9.6) for 1: 10 ~ 4 diluted goat anti-mouse IgG antiserum overnight at 4 ° C, washed three times with PBS-Tween containing 0.05% Tween; each sensitized bead was placed on a microtiter plate of 1