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目的:探讨超滤-反渗透浓缩黄芩水提液的可行性。方法:黄芩水提液经离心、超滤及反渗透处理,通过HPLC特征图谱考察处理前后黄芩水提液中主要化学组成的变化,流动相甲醇(A)-0.05%磷酸水溶液(B)梯度洗脱(0~35 min,40%A;35~75 min,60%A),检测波长280 nm。考察反渗透膜的清洗液(碱液、酸液、十二烷基苯磺酸钠溶液及纯水)。结果:浓缩4 h后,经预处理的药液浓缩倍数为3.2倍,通量衰减了67.64%;未经预处理的药液浓缩倍数为1.6倍,通量衰减了85.35%。浓缩后药液中黄芩苷质量浓度由1.03 g·L-1增至8.87 g·L-1。各试液相似度均>0.99,说明浓缩前后样品的相似性良好。采用不同清洗液清洗时,反渗透膜的通量恢复率依次为87.73%,81.50%,67.65%,93.35%,选择纯水清洗反渗透膜。结论:超滤-反渗透集成工艺化学成分保留率、浓缩效率高,适用于黄芩水提液的有效浓缩。
Objective: To explore the feasibility of ultrafiltration - reverse osmosis concentrated Scutellaria water extract. Methods: The extracts of Scutellaria baicalensis were separated by centrifugation, ultrafiltration and reverse osmosis. The main chemical composition of Scutellariae Scutellariae before and after treatment was investigated by HPLC chromatogram. The mobile phase of methanol (A) -0.05% phosphoric acid aqueous solution (B) Off (0-35 min, 40% A; 35-75 min, 60% A) at a detection wavelength of 280 nm. Investigate the reverse osmosis membrane cleaning solution (lye, acid, sodium dodecyl benzene sulfonate solution and pure water). Results: After 4 h of concentration, the concentration of the pretreated liquid was 3.2 times and the flux was reduced by 67.64%. The concentration of non-pretreated liquid was 1.6 times and the flux decayed by 85.35%. The concentration of baicalin in the concentrated solution increased from 1.03 g · L-1 to 8.87 g · L-1. The similarity of each test solution were> 0.99, indicating that the similarity of samples before and after concentration was good. When different cleaning solutions were used, the flux recovery rates of reverse osmosis membranes were 87.73%, 81.50%, 67.65% and 93.35%, respectively. The reverse osmosis membranes were cleaned with pure water. Conclusion: Ultrafiltration - reverse osmosis integrated process chemical composition retention rate, high concentration efficiency, suitable for the effective concentration of Scutellaria water extract.