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目的 以体外原代培养的皮层神经元为对象 ,研究电磁脉冲 (场强为 6× 10 4 V m)辐射后早期神经细胞死亡和凋亡的变化情况 ,并探讨电磁脉冲的可能致伤机制。方法 于照射后 1h、6h、12h、2 4h和 48h分别采用MTT比色法和流式细胞术测定细胞活力和凋亡细胞的比例 ,用HE染色及TUNEL检测细胞凋亡并分别用普通光学显微镜和萤光显微镜进行凋亡观察。结果 电磁脉冲辐射后 ,神经细胞不仅发生快速的坏死 ,而且还发生细胞凋亡 ,于辐射作用后 12h达到高峰。结论 电磁脉冲辐射后早期可导致神经细胞凋亡和坏死 ,此改变可能与电磁脉冲致细胞DNA损伤有关。
OBJECTIVE: To investigate the changes of neuronal cell death and apoptosis after radiation of electromagnetic pulse (field intensity of 6 × 10 4 V m) in vitro and to investigate the possible mechanism of electromagnetic pulse injury. Methods The viability and the percentage of apoptotic cells were determined by MTT assay and flow cytometry respectively at 1h, 6h, 12h, 24h and 48h after irradiation. The apoptosis of the cells was detected by HE staining and TUNEL. The cells were harvested with ordinary light microscope And fluorescence microscope observation of apoptosis. Results Electromagnetic pulse radiation, the nerve cells not only rapid necrosis, but also the occurrence of apoptosis, peaked at 12h after irradiation. Conclusion Electromagnetic pulse radiation can lead to apoptosis and necrosis of neural cells in early stage, which may be related to DNA damage caused by electromagnetic pulse.