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目的:通过不同造模方法的比较来建立新的高脂血症造模方法。方法:对四组小鼠分别进行正常喂养、乳剂灌胃、高脂饲料喂养和在高脂饲料喂养的同时隔天乳剂灌胃。然后在第0,7,14,21天检测小鼠血压以及血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)含量,并在实验结束时测定脏器系数。结果:乳剂+高脂组肝系数显著高于其他组;血压值在14 d后显著升高(P<0.01);TC含量和TG含量分别在7 d和14 d后显著升高(P<0.01);HDL-C含量在7 d后显著性降低(P<0.01);第21天与第14天相比,血压和血脂水平能够继续维持,并且乳剂+高脂组较乳剂灌胃组小鼠死亡率低。结论:本造模方法切实可行,在短期内效果明显。
Objective: To establish a new hyperlipemia modeling method by comparing different modeling methods. Methods: Four groups of mice were fed with normal diet, intragastric administration of milk, high-fat diet and high-fat diet while feeding the emulsion to the other day. Mice were then tested for blood pressure and serum total cholesterol (TC), triglyceride (TG) and high density lipoprotein cholesterol (HDL-C) levels on days 0, 7, coefficient. Results: The liver coefficient of the emulsion + hyperlipidemic group was significantly higher than that of the other groups. The blood pressure increased significantly after 14 days (P <0.01). The contents of TC and TG were significantly increased at 7 and 14 d ); HDL-C content decreased significantly after 7 days (P <0.01); on the 21st day compared with the 14th day, blood pressure and blood lipid level could be maintained, and the emulsion + Low mortality. Conclusion: The modeling method is feasible and effective in the short term.