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目的采用可见光持续间断光照建立广西巴马猪的慢性视网膜变性模型,并探索活体鉴定广西巴马猪视网膜功能的方法。方法白色可见光持续间断照射3~4月龄广西巴马小型猪3~6个月,建立小型猪视网膜变性模型。通过对模型猪进行眼底照相、光学相干断层扫描(OCT)、全视野视网膜电图(fERG)和多焦视网膜电图(mfERG)等检测,从形态和功能对光诱导视网膜变性模型进行活体鉴定。结果与光照前相比,光变性巴马小型猪眼底照相无显著可见改变;OCT可见光变性猪视网膜ILM~RPE层厚度显著变薄(P<0.05);fERG可见光变性猪在暗适应时最大混合反应的a、b波和ops波振幅均有显著下降(P<0.05),在明适应时b波及P1波波幅也有显著下降(P<0.05);mfERG可见光变性猪P1波平均振幅密度显著降低(P<0.05)。结论可见光持续间断光照可诱导广西巴马小型猪视网膜出现结构和功能改变,可作为慢性视网膜变性模型。
OBJECTIVE: To establish a chronic retinal degeneration model of Guangxi Bama pig by continuous light interruption of visible light and to explore the method of identifying the retinal function of Guangxi Bama pig in vivo. Methods The white visible light was irradiated intermittently for 3 to 4 months in Guangxi Bama miniature pigs for 3 to 6 months to establish mini pig retinal degeneration model. The model pigs were examined by fundus photography, optical coherence tomography (OCT), full-field fERG and mfERG, and the morphological and functional models were used for the in vivo identification of light-induced retinal degeneration. Results Compared with those before light exposure, there was no significant change in fundus photography of light-attenuated Bama miniature pigs. Thickness of ILM-RPE layer in porcine retina with OCT visible light degeneration was significantly thinner (P <0.05). The maximal mixed reaction of fERG visible light- (P <0.05). The amplitudes of b wave and P1 wave were also significantly decreased (P <0.05) at Ming adaptation, and the average amplitudes of P1 wave in mfERG visible light mutant pig were significantly decreased (P <0.05). CONCLUSION: The continuous light interruption of visible light can induce the changes of retinal structure and function in Guangxi Bama miniature pigs, which can be used as a model of chronic retinal degeneration.